0093. Mitochondrial function of immune cells in severe sepsis and septic shock - a prospective observational cohort study

Methods 30 patients with severe sepsis or septic shock were studied at ICU admission and after 24 and 48 hours. Immune cells were identified and isolated using an immunomagnetic positive cell isolation procedure (Dynabeads for Human Monocytes, Human B cells, Human CD4 cells, Invitrogen Dynal AS, Oslo, Norway). Enzymatic activity of mitochondrial complexes I, IV, and ATP synthase and ATP content were measured spectrophotometrically and expressed as ratio to citrate synthase for enzymatic activities to account for mitochondrial mass and as mass per μg of cellular protein for ATP content. Maximal mitochondrial enzymatic activities and ATP in the first 48h of sepsis were compared with samples from 20 healthy volunteers (Mann Whitney test).